MtnA

• Description: 5-methylthioribose-1-phosphate isomerase
  
  

Gene name	mtnA
Synonyms	ykrS, mtnS
Essential	no
Product	5-methylthioribose-1-phosphate isomerase
Function	methionine salvage
Gene expression levels in SubtiExpress: mtnA
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	38 kDa, 5.003
Gene length, protein length	1059 bp, 353 aa
Immediate neighbours	ogt, mtnK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, phosphoproteins

This gene is a member of the following regulons

S-box

The gene

Basic information

• Locus tag: BSU13550

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: S-methyl-5-thio-alpha-D-ribose 1-phosphate = S-methyl-5-thio-D-ribulose 1-phosphate (according to Swiss-Prot)

• Protein family: MtnA subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-80 and Arg-298 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2YVK

• UniProt: O31662

• KEGG entry: [3]

• E.C. number: 5.3.1.23

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon: mtnK-mtnA PubMed

• Expression browser: mtnA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by methionine starvation (S-box) PubMed
  • repressed by casamino acids PubMed

• Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: