YbgE

• Description: branched-chain amino acid aminotransferase
  
  

Gene name	ybgE
Synonyms
Essential	no
Product	branched-chain amino acid aminotransferase
Function	biosynthesis of branched-chain amino acids
Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis, Ile, Leu, Val, Coenzyme A
MW, pI	39 kDa, 4.952
Gene length, protein length	1068 bp, 356 aa
Immediate neighbours	ybgB, ybgF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

• Locus tag: BSU02390

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: l-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate (according to Swiss-Prot)

• Protein family: class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)

• Paralogous protein(s): YwaA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O31461

• KEGG entry: [3]

• E.C. number: 2.6.1.42

Expression and regulation

• Operon: ybgE PubMed

• Sigma factor:

• Regulation:
  • repressed by casamino acids PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • a ybgE mutant and a bcd ybgE ywaA triple mutant are available in Linc Sonenshein's lab

• Expression vector:

• lacZ fusion: pGP248 (in pAC5), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody: