Difference between revisions of "Hag"
(→Extended information on the protein) |
|||
Line 121: | Line 121: | ||
* '''Additional information:''' | * '''Additional information:''' | ||
+ | ** the ''[[hag]]'' gene is strongly overexpressed in a ''[[ymdB]]'' mutant (loss of bistable gene expression) {{PubMed|21856853}} | ||
=Biological materials = | =Biological materials = | ||
Line 141: | Line 142: | ||
=References= | =References= | ||
+ | <big>''Diethmaier C, Pietack N, Gunka K, Wrede C, Lehnik-Habrink M, Herzberg C, Hübner S, Stülke J'' </big> | ||
+ | <big>'''A Novel Factor Controlling Bistability in ''Bacillus subtilis'': The YmdB Protein Affects</big> | ||
+ | <big>Flagellin Expression and Biofilm Formation.''' </big> | ||
+ | J Bacteriol. 2011 Aug 19. [Epub ahead of print] | ||
+ | [http://www.ncbi.nlm.nih.gov/pubmed/21856853 PubMed:21856853] | ||
<pubmed>2498284,9648743,9096206,7708689,19202088, 18763711, 19118355 17555441 10809682 20534509 21602220 </pubmed> | <pubmed>2498284,9648743,9096206,7708689,19202088, 18763711, 19118355 17555441 10809682 20534509 21602220 </pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] |
Revision as of 11:35, 23 August 2011
- Description: flagellin protein
Gene name | hag |
Synonyms | |
Essential | no |
Product | flagellin protein |
Function | movement and chemotaxis |
Interactions involving this protein in SubtInteract: Hag | |
MW, pI | 32 kDa, 4.782 |
Gene length, protein length | 912 bp, 304 aa |
Immediate neighbours | yvyC, csrA |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
CodY regulon, CsrA regulon, ScoC regulon, SigD regulon
The gene
Basic information
- Locus tag: BSU35360
Phenotypes of a mutant
No swarming motility on B medium. PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: bacterial flagellin family (according to Swiss-Prot)
- Paralogous protein(s): YvzB (C-terminal domain of Hag)
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P02968
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP901 (aphA3), GP902 (tet), both available in Stülke lab
- Expression vector:
- lacZ fusion: pGP1035 (in pAC6), pGP755 (in pAC7), there is also a series of promoter deletion variants in pAC6 and pAC7, all available in Stülke lab
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Diethmaier C, Pietack N, Gunka K, Wrede C, Lehnik-Habrink M, Herzberg C, Hübner S, Stülke J A Novel Factor Controlling Bistability in Bacillus subtilis: The YmdB Protein Affects Flagellin Expression and Biofilm Formation. J Bacteriol. 2011 Aug 19. [Epub ahead of print] PubMed:21856853