Difference between revisions of "SigL"
Line 30: | Line 30: | ||
<br/><br/><br/> | <br/><br/><br/> | ||
+ | |||
+ | = [[Categories]] containing this gene/protein = | ||
+ | {{SubtiWiki category|[[transcription]]}}, | ||
+ | {{SubtiWiki category|[[sigma factors and their control]]}} | ||
+ | |||
+ | = This gene is a member of the following [[regulons]] = | ||
+ | {{SubtiWiki regulon|[[CcpA regulon]]}} | ||
=The gene= | =The gene= | ||
Line 50: | Line 57: | ||
− | + | ||
− | |||
− | |||
=The protein= | =The protein= | ||
Revision as of 23:32, 8 December 2010
- Description: sigma factor of the RNA polymerase, Sigma-54, Sigma L
Gene name | sigL |
Synonyms | |
Essential | no |
Product | RNA polymerase sigma-54 factor (sigma-L) |
Function | utilization of arginin, acetoin and fructose, required for cold adaptation |
MW, pI | 49,5 kDa, 7.79 |
Gene length, protein length | 1308 bp, 436 amino acids |
Immediate neighbours | yvfH, yvfG |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
Categories containing this gene/protein
transcription, sigma factors and their control
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU34200
Phenotypes of a mutant
The mutant is cold-sensitive and unable to use arginine as a single carbon source. PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Binding to promoters of the -12, -24 type
- Protein family: sigma-54 factor family (according to Swiss-Prot) sigma-54 factor family
- Paralogous protein(s):
Genes/ operons controlled by SigL
acoA-acoB-acoC-acoL, rocA-rocB-rocC, rocD-rocE-rocF, rocG, levD-levE-levF-levG-sacC, ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB
Transcription factors activating transcription at SigL-dependent promoters
Extended information on the protein
- Kinetic information:
- Domains:
- DNA binding domain (H-T-H motif) (324–343)
- pron box domain (413–421)
- 3 x Compositional bias domain (6–21),(32–53),(112–136)
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure:
- UniProt: P24219
- KEGG entry: [3]
Additional information
Transcription initiation by SigL-containing RNA polymerase requires the activity of ATP-hydrolyzing transcription activators.
Transcription factors activating transcription at SigL-dependent promoters
Expression and regulation
- Operon: sigL (according to DBTBS)
- Additional information:
Biological materials
- Mutant: GP146 (aphA3), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- Antibody:
Labs working on this gene/protein
Michel Debarbouille, Pasteur Institute, Paris, France Homepage
Your additional remarks
References
Reviews
Original publications