Difference between revisions of "NrdF"
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' ''[[nrdI]]-[[nrdE]]-[[nrdF]]-[[ymaB]]'' | + | * '''Operon:''' ''[[nrdI]]-[[nrdE]]-[[nrdF]]-[[ymaB]]'' {{PubMed|16885274}} |
| − | * '''[[Sigma factor]]:''' | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|16885274}} |
| − | * '''Regulation:''' | + | * '''Regulation:''' |
| − | ** expressed under anaerobic conditions ([[ResD]]) | + | ** expressed under anaerobic conditions ([[ResD]]) {{PubMed|16885274}} |
| − | * '''Regulatory mechanism:''' | + | * '''Regulatory mechanism:''' |
| − | ** [[ResD]]: transcription activation | + | ** [[ResD]]: transcription activation {{PubMed|16885274}} |
| − | * '''Additional information:''' | + | * '''Additional information:''' |
=Biological materials = | =Biological materials = | ||
Revision as of 20:18, 16 October 2009
- Description: ribonucleoside-diphosphate reductase (major subunit)
| Gene name | nrdF |
| Synonyms | |
| Essential | yes PubMed |
| Product | ribonucleoside-diphosphate reductase (major subunit) |
| Function | synthesis of deoxyribonucleoside triphosphates |
| Metabolic function and regulation of this protein in SubtiPathways: Purine salvage, Nucleotides (regulation) | |
| MW, pI | 38 kDa, 4.66 |
| Gene length, protein length | 987 bp, 329 aa |
| Immediate neighbours | nrdE, ymaB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
The gene
Basic information
- Locus tag: BSU17390
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin (according to Swiss-Prot)
- Protein family: ribonucleoside diphosphate reductase small chain family (according to Swiss-Prot)
- Paralogous protein(s): YosP
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure:
- UniProt: P50621
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Elisabeth Härtig, Braunschweig, Germany Homepage
Your additional remarks
References
