SigM

• Description: RNA polymerase ECF-type sigma factor SigM
  
  

Gene name	sigM
Synonyms	yhdM
Essential	no
Product	RNA polymerase ECF-type sigma factor SigM
Function	resistance against cell envelope stress, oxidative stress and salt stress
MW, pI	19 kDa, 6.77
Gene length, protein length	489 bp, 163 aa
Immediate neighbours	yhdL, yhdN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU09520

Phenotypes of a mutant

• SigM is essential for growth and survival in nutrient broth (NB) containing 1.4 M NaCl PubMed
• sigM mutants form aberrantly shaped cells, which swell and lyse spontaneously during growth in NB medium containing increased levels (0.35-0.7 M) of a wide range of different salts PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: sigma-70 factor family, ECF subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Genes/ operons controlled by SigM

abh, divIC, sigM-yhdLyhdK, yqjL, ypbG, ydaH, ypuA, radC, yebC, ywtF, rodA, ypuD, ytpA, ytpB, yngC, ycgR, ycgQ

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: SigM-YhdK-YhdL

• Localization:

Database entries

• Structure:

• UniProt: O07582

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sigM-yhdL-yhdK PubMed

• Sigma factor: SigA PubMed, SigM PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage