TagT

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  • Description: phosphotransferase, attaches teichoic acid, teichuronic acid, or acidic capsular polysaccharide to cell wall peptidoglycan via phosphodiester linkage to N-acteylmuramic acid

Gene name ywtF
Synonyms
Essential no
Product phosphotransferase, responsible for attachment of anionic polymers to peptidoglycan
Function transfer of anionic cell wall polymers from lipid-linked precursors to peptidoglycan
Gene expression levels in SubtiExpress: tagT
Interactions involving this protein in SubtInteract: TagT
MW, pI 35 kDa, 9.554
Gene length, protein length 966 bp, 322 aa
Immediate neighbours ywtG, ywtE
Sequences Protein DNA DNA_with_flanks
Genetic context
YwtF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
TagT expression.png















Categories containing this gene/protein

cell wall synthesis, cell envelope stress proteins (controlled by SigM, V, W, X, Y), membrane proteins

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

  • Locus tag: BSU35840

Phenotypes of a mutant

  • a tagT mutant has no phenoype, the triple tagT tagU tagV mutant is unable to grow under normal conditions PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: LytR-Cps2a-Psr family PubMed

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Effectors of protein activity:
  • Localization:
    • cytoplasmic membrane (with extracytoplasmic catalaytic domain) PubMed
    • extracellular (signal peptide) PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed at high salt concentrations (SigM) Pubmed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 105 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 258 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications