PstBA

• Description: phosphate ABC transporter (ATP-binding protein)
  
  

Gene name	pstBA
Synonyms	pstB, yqgJ, yzmE
Essential	no
Product	phosphate ABC transporter (ATP-binding protein)
Function	high-affinity phosphate uptake
MW, pI	29 kDa, 7.897
Gene length, protein length	807 bp, 269 aa
Immediate neighbours	pstBB, pstA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU24960

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + H₂O + phosphate(Out) = ADP + phosphate + phosphate(In) (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: P46341

• KEGG entry: [2]

• E.C. number: 3.6.3.27

Expression and regulation

• Operon: pstS-pstC-pstA-pstBA-pstBB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions of phosphate limitation (5000-fold induced) (PhoP) PubMed

• Regulatory mechanism:
  • PhoP-P: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Marion Hulett, University of Illinois at Chicago, USA Homepage

References

W Liu, Y Qi, F M Hulett
Sites internal to the coding regions of phoA and pstS bind PhoP and are required for full promoter activity.
Mol Microbiol: 1998, 28(1);119-30
[PubMed:9593301] [WorldCat.org] [DOI] (P p)

Y Qi, Y Kobayashi, F M Hulett
The pst operon of Bacillus subtilis has a phosphate-regulated promoter and is involved in phosphate transport but not in regulation of the pho regulon.
J Bacteriol: 1997, 179(8);2534-9
[PubMed:9098050] [WorldCat.org] [DOI] (P p)

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed