GltA

• Description: large subunit of glutamate synthase, small subunit is gltB, glutamate biosynthesis is induced by sugar and repressed by arginine
  
  

Gene name	gltA
Synonyms
Essential	no
Product	glutamate synthase (large subunit)
Function	glutamate biosynthesis
MW, pI	168 kDa, 5.47
Gene length, protein length	4560 bp, 1520 amino acids
Immediate neighbours	gltC, gltB
Gene sequence (+200bp)	Protein sequence
Genetic context File:GenE context.gif

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2 L-glutamate + NADP(+) <=> L-glutamine + 2-oxoglutarate + NADPH

• Protein family: glutamate synthase family

• Paralogous protein(s): YerD

Extended information on the protein

• Kinetic information:

• Domains:
  • Glutamine amidotransferase type-2 domain (22-415)
  • Nucleotide binding domain (1060-1112)

• Modification:

• Cofactor(s): 3Fe-4S, FAD, FMN

• Effectors of protein activity:

• Interactions:

• Localization: membrane protein

Database entries

• Structure:

• Swiss prot entry: [3]

• KEGG entry: [4]

• E.C. number: [5]

Additional information

subject to Clp-dependent proteolysis upon glucose starvation

Expression and regulation

• Operon: gltAB

• Sigma factor: SigA

• Regulation: induced by sugar, repressed by arginine, ammonium required

• Regulatory mechanism: Aktivator: GltC; Repressor: TnrA

• Additional information:

Labs working on this gene/protein

Linc Sonenshein, Tufts University, Boston, MA, USA Homepage

Jörg Stülke, University of Göttingen, Germany Homepage

References

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed

1. Yoshida K, et al. (2003) Journal volume: page-page. PubMed