CysK

• Description: trigger enzyme: cysteine synthetase A
  
  

Gene name	cysK
Synonyms
Essential	no
Product	cysteine synthetase A
Function	biosynthesis of cysteine, control of CymR activity
MW, pI	32 kDa, 5.492
Gene length, protein length	924 bp, 308 aa
Immediate neighbours	yacD, pabB
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Phenotypes of a mutant

constitutive expression of the cysH operon, auxotrophic for cysteine

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: CymR-CysK, the complex is formed in the presence of cysteine, and results in DNA binding and repression of genes of cysteine biosynthesis PubMed

• Localization:

Database entries

• Structure:

• Swiss prot entry:

• KEGG entry: [3]

• E.C. number: EC 4.2.99.8

Expression and regulation

• Operon: cysK

• Sigma factor: SigA

• Regulation: repressed by casamino acids PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Mäder et al. (2002) Transcriptome and Proteome Analysis of Bacillus subtilis Gene Expression Modulated by Amino Acid Availability. J. Bacteriol 184: 1844288-4295 PubMed
2. Tanous et al. (2008) The CymR regulator in complex with the enzyme CysK controls cysteine metabolism in Bacillus subtilis.J. Biol. Chem. 283: 35551-35560. PubMed
3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed