LeuB

• Description: 3-isopropylmalate dehydrogenase
  
  

Gene name	leuB
Synonyms	leuC
Essential	no
Product	3-isopropylmalate dehydrogenase
Function	biosynthesis of leucine
MW, pI	39 kDa, 4.744
Gene length, protein length	1095 bp, 365 aa
Immediate neighbours	leuC, leuA
Gene sequence (+200bp)	Protein sequence
Caution: The sequence for this gene in SubtiList contains errors
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry:

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ilvB-ilvH-ilvC-leuA-leuB-leuC-leuD

• Sigma factor: SigA

• Regulation: expressed in the absence of branched-chain amino acids (BCAA), expression is stimulated in the presence of glucose PubMed

• Regulatory mechanism: glucose regulation: CcpA PubMed, repression by BCAA: tRNA-controlled RNA switch (T-box) that mediates termination/antitermination, transcription repression by CodY

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed