OppA

• Description: oligopeptide ABC transporter (binding protein)
  
  

Gene name	oppA
Synonyms	spo0KA
Essential	no
Product	oligopeptide ABC transporter (binding protein)
Function	initiation of sporulation, competence development
Gene expression levels in SubtiExpress: oppA
Interactions involving this protein in SubtInteract: OppA
Metabolic function and regulation of this protein in SubtiPathways: OppA
MW, pI	61 kDa, 5.722
Gene length, protein length	1635 bp, 545 aa
Immediate neighbours	trpS, oppB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, utilization of nitrogen sources other than amino acids, genetic competence, phosphorelay, membrane proteins

This gene is a member of the following regulons

CodY regulon, ScoC regulon, TnrA regulon

The gene

Basic information

• Locus tag: BSU11430

Database entries

• BsubCyc: BSU11430

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • required for the uptake of quorum sensing peptides such as PhrH PubMed

• Protein family: bacterial solute-binding protein 5 family (according to Swiss-Prot)

• Paralogous protein(s): DppE

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on (Tyr-301 OR Tyr-303) AND Thr-470 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • (OppD-OppF)-(OppB-OppC)-OppA PubMed
  • FloT-OppA PubMed

• Localization:
  • attached to the cell membrane (via OppB-OppC) PubMed
  • at the outer side of the cell: extracellular (signal peptide) PubMed, lipid modification as retention signal PubMed

Database entries

• BsubCyc: BSU11430

• Structure:

• UniProt: P24141

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: oppA-oppB-oppC-oppD-oppF PubMed

• Expression browser: oppA PubMed

• Sigma factor:

• Regulation:
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • repressed during exponential growth (ScoC) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed
  • ScoC: transcription repression PubMed
  • CodY: transcription repression, no derepression occurs, however, in a codY mutant, due to increased repression by ScoC PubMed

• Additional information:
  • A ncRNA is predicted between trpS and oppA PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1937 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 20139 PubMed

Biological materials

• Mutant:
  • BP67 (spc) available in Fabian Commichau's lab
  • 1S118 ( oppA::spec), PubMed, available at BGSC
  • GP2097 (D(oppA-oppB-oppC-oppD-oppF)::aphA3), available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: