YqhY

• Description: unknown
  
  

Gene name	yqhY
Synonyms
Essential	no PubMed
Product	unknown
Function	unknown
Gene expression levels in SubtiExpress: yqhY
Metabolic function and regulation of this protein in SubtiPathways: yqhY
MW, pI	14 kDa, 4.414
Gene length, protein length	405 bp, 135 aa
Immediate neighbours	nusB, accC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

proteins of unknown function

The gene

Basic information

• Locus tag: BSU24330

Database entries

• BsubCyc: BSU24330

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: asp23 family (according to Swiss-Prot)

• Paralogous protein(s): YloU

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU24330

• Structure:

• UniProt: P54519

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: accB-accC-yqhY PubMed

• Expression browser: yqhY PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 127 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 893 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 4905 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 3644 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 4928 PubMed

Biological materials

• Mutant:
  • GP1468 (yqhY::erm), available in Jörg Stülke's lab PubMed
  • GP1765 (yqhY::cat), available in Jörg Stülke's lab PubMed
  • GP1474 (chromosomal yqhY-Strep fusion, aphA3), purification from B. subtilis, for SPINE, available in Jörg Stülke's lab

• Expression vector:
  • pGP1322 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
  • pGP1325 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
  • pGP1496 (His-tag, purification from E. coli, in pET28a+), available in Jörg Stülke's lab
  • pGP1498 (N-terminal His-tag, TEV-site, purification from E. coli, in pWH844), available in Jörg Stülke's lab

• lacZ fusion:

• GFP fusion:
  • GP1471 (spc, based on pBP43), available in Jörg Stülke's lab

• YFP fusion:
  • GP1492 (cat, available in Jörg Stülke's lab

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

• FLAG-tag construct:
  • GP1481 (spc, based on pGP1331), available in Jörg Stülke's lab

• Antibody: