rfaA

rfaA
168

modulator of lipid biosynthesis

Locus
BSU_24330
Molecular weight
14.55 kDa
Isoelectric point
4.41
Protein length
135 aaSequenceBlast
Gene length
408 bpSequenceBlast
Function
control of fatty acid biosynthesis
Product
modulator of lipid biosynthesis
Essential
quasi
Synonyms
yqhY, yqhY
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1302 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,529,926 → 2,530,333
Phenotypes of a mutant
the mutant readily acquires suppressor mutants that result in reduced activity of the ACCase PubMed
the mutant tends to acquire suppressor mutations that result in improved growth PubMed
enhanced colony wrinkling upon knockdown PubMed
the mutant forms lipophilic clusters PubMed
severe growth inhibition upon addition of subinhibitory concentrations of mirubactin C PubMed
The protein
Protein family
asp23 family (with RfaB, according to UniProt)
Structure
P54519 (AlphaFold)
Paralogous protein(s)
RfaB
Localization
cell poles PubMed
Expression and Regulation
Operons
Genes
accB-accC-rfaA
Description
PubMed
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accB→rfaA

2025-03-30 02:37:29

ghost

163

86e257ca959326e8b837637c9b51bc6f18c4c8c4

C4E0BC3C47DE59CA027C5A57E3491771B42AECD9

Biological materials
Mutant
MGNA-C367 (yqhY::erm), available at the NBRP B. subtilis, Japan
GP1468 (ΔyqhY::erm), available in Jörg Stülke's lab PubMed
GP1765 (ΔyqhY::cat), available in Jörg Stülke's lab PubMed
BKE24330 (ΔrfaA ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCAATTCACCTCCGTAA,  downstream forward: _UP4_TAAATGGCTTAACACGAAAC
BKK24330 (ΔrfaA ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCAATTCACCTCCGTAA,  downstream forward: _UP4_TAAATGGCTTAACACGAAAC
Expression vectors
GP1474 (chromosomal yqhY-Strep fusion, aphA3), purification from B. subtilis, for SPINE, available in Jörg Stülke's lab
pGP1322 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
pGP1325 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
pGP1496 (His-tag, purification from E. coli, in pET28a+), available in Jörg Stülke's lab
pGP1498 (N-terminal His-tag, TEV-site, purification from E. coli, in pWH844), available in Jörg Stülke's lab
pGP3619 expression of rfaA -Strep by pGP382 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
pGP3630 (N-terminal 6xHis-SUMO-tag, purification from E. coli, in pET-SUMO), available in Jörg Stülke's lab
pGP3644 (N-terminal GST-Tag, purification from E. coli, in pGEX-6P-1), available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
FLAG-tag construct
GP1481 (spc, based on pGP1331), available in Jörg Stülke's lab
GFP fusion
GP1471 (spc, based on pBP43), available in Jörg Stülke's lab PubMed
References
Reviews
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Original Publications
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Time of last update: 2025-04-01 09:23:13

Author of last update: Jstuelk