YrvO

• Description: cysteine desulfurase involved in 2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules
  
  

Gene name	yrvO
Synonyms	nifS, iscS
Essential	yes PubMed
Product	cysteine desulfurase
Function	tRNA modification
Gene expression levels in SubtiExpress: yrvO
MW, pI	41 kDa, 5.463
Gene length, protein length	1137 bp, 379 aa
Immediate neighbours	trmU, cymR
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

essential genes, translation, phosphoproteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU27510

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU27510

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • 2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules (together with MnmA) PubMed

• Protein family:

• Paralogous protein(s): NifS, NifZ, SufS, YcbU

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-385 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU27510

• Structure: 1EG5 (from Thermotoga maritima, 37% identity, 54% similarity) PubMed

• UniProt: O34599

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cymR-yrvO-trmU PubMed

• Expression browser: yrvO PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 184 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 444 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: