BsrA

• Description: 6S RNA, important for timing of sporulation
  
  

Gene name	bsrA
Synonyms
Essential	no
Product	6S RNA
Function	antagonist of SigA
Interactions involving this RNA in SubtInteract: BsrA
Gene length	190 bp
Immediate neighbours	yrvM, aspS
Gene sequence (+200bp)
Genetic context

Categories containing this gene/protein

sigma factors and their control, ncRNA

The gene

Basic information

• Locus tag: BSU_misc_RNA_41

Phenotypes of a mutant

• reduced growth rate PubMed
• delayed outgrowth from stationary phase PubMed
• altered timing of onset of sporulation (earlier sporulation) PubMed

Database entries

• BsubCyc: BSU_misc_RNA_41

• DBTBS entry: no entry

• SubtiList entry: no entry

• Structure:

Additional information

• Similar RNA: bsrB

• Interactions: BsrA-SigA-RNA polymerase

Expression and regulation

• Operon: bsrA PubMed

• Sigma factor: SigA PubMed

• Regulation: expressed in vegetative cells PubMed

• Regulatory mechanism:

• Additional information: transcribed as 201 nt RNA, processed to mature 190 bp 6S RNA PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

Labs working on this gene/RNA

Kouji Nakamura, Tsukuba, University, Japan

Roland Hartmann, Philipps-Universität Marburg, Marburg, Germany, Homepage

References

Reviews

Stefanie Wehner, Katrin Damm, Roland K Hartmann, Manja Marz
Dissemination of 6S RNA among bacteria.
RNA Biol: 2014, 11(11);1467-78
[PubMed:25483037] [WorldCat.org] [DOI] (I p)

Benedikt Steuten, Philipp G Hoch, Katrin Damm, Sabine Schneider, Karen Köhler, Rolf Wagner, Roland K Hartmann
Regulation of transcription by 6S RNAs: insights from the Escherichia coli and Bacillus subtilis model systems.
RNA Biol: 2014, 11(5);508-21
[PubMed:24786589] [WorldCat.org] [DOI] (I p)

Amy T Cavanagh, Karen M Wassarman
6S RNA, a global regulator of transcription in Escherichia coli, Bacillus subtilis, and beyond.
Annu Rev Microbiol: 2014, 68;45-60
[PubMed:24742053] [WorldCat.org] [DOI] (I p)

Benedikt Steuten, Sabine Schneider, Rolf Wagner
6S RNA: recent answers--future questions.
Mol Microbiol: 2014, 91(4);641-8
[PubMed:24308327] [WorldCat.org] [DOI] (I p)

Original publications

Olga Y Burenina, Philipp G Hoch, Katrin Damm, Margarita Salas, Timofei S Zatsepin, Marcus Lechner, Tatiana S Oretskaya, Elena A Kubareva, Roland K Hartmann
Mechanistic comparison of Bacillus subtilis 6S-1 and 6S-2 RNAs--commonalities and differences.
RNA: 2014, 20(3);348-59
[PubMed:24464747] [WorldCat.org] [DOI] (I p)

Ignacio J Cabrera-Ostertag, Amy T Cavanagh, Karen M Wassarman
Initiating nucleotide identity determines efficiency of RNA synthesis from 6S RNA templates in Bacillus subtilis but not Escherichia coli.
Nucleic Acids Res: 2013, 41(15);7501-11
[PubMed:23761441] [WorldCat.org] [DOI] (I p)

Amy T Cavanagh, Karen M Wassarman
6S-1 RNA function leads to a delay in sporulation in Bacillus subtilis.
J Bacteriol: 2013, 195(9);2079-86
[PubMed:23457253] [WorldCat.org] [DOI] (I p)

Benedikt M Beckmann, Philipp G Hoch, Manja Marz, Dagmar K Willkomm, Margarita Salas, Roland K Hartmann
A pRNA-induced structural rearrangement triggers 6S-1 RNA release from RNA polymerase in Bacillus subtilis.
EMBO J: 2012, 31(7);1727-38
[PubMed:22333917] [WorldCat.org] [DOI] (I p)

Amy T Cavanagh, Jamie M Sperger, Karen M Wassarman
Regulation of 6S RNA by pRNA synthesis is required for efficient recovery from stationary phase in E. coli and B. subtilis.
Nucleic Acids Res: 2012, 40(5);2234-46
[PubMed:22102588] [WorldCat.org] [DOI] (I p)

Benedikt M Beckmann, Olga Y Burenina, Philipp G Hoch, Elena A Kubareva, Cynthia M Sharma, Roland K Hartmann
In vivo and in vitro analysis of 6S RNA-templated short transcripts in Bacillus subtilis.
RNA Biol: 2011, 8(5);839-49
[PubMed:21881410] [WorldCat.org] [DOI] (I p)

Jeffrey E Barrick, Narasimhan Sudarsan, Zasha Weinberg, Walter L Ruzzo, Ronald R Breaker
6S RNA is a widespread regulator of eubacterial RNA polymerase that resembles an open promoter.
RNA: 2005, 11(5);774-84
[PubMed:15811922] [WorldCat.org] [DOI] (P p)

Satoru Suzuma, Sayaka Asari, Keigo Bunai, Keiko Yoshino, Yoshinari Ando, Hiroshi Kakeshita, Masaya Fujita, Kouji Nakamura, Kunio Yamane
Identification and characterization of novel small RNAs in the aspS-yrvM intergenic region of the Bacillus subtilis genome.
Microbiology (Reading): 2002, 148(Pt 8);2591-2598
[PubMed:12177353] [WorldCat.org] [DOI] (P p)