LytF

• Description: gamma-D-glutamate-meso-diaminopimelate muropeptidase, cell separase (major autolysin)
  
  

Gene name	lytF
Synonyms	cwlE, yhdD
Essential	no
Product	gamma-D-glutamate-meso-diaminopimelate muropeptidase (major autolysin)
Function	cell separation
Gene expression levels in SubtiExpress: lytF
Interactions involving this protein in SubtInteract: LytF
Metabolic function and regulation of this protein in SubtiPathways: LytF
MW, pI	51 kDa, 10.41
Gene length, protein length	1464 bp, 488 aa
Immediate neighbours	yhdC, nsrR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover

This gene is a member of the following regulons

SigD regulon, SlrR regulon

The gene

Basic information

• Locus tag: BSU09370

Phenotypes of a mutant

• cell separation defect, this is increased by a lytE mutation PubMed

Database entries

• BsubCyc: BSU09370

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: nlpC/p60 family (according to Swiss-Prot)

• Paralogous protein(s): the C-terminal D,L-endopeptidase domains of LytE, LytF, CwlS, and CwlO exhibit strong sequence similarity

Extended information on the protein

• Kinetic information:

• Domains:
  • contains five N-acetylglucosamine-polymer-binding LysM domains PubMed
  • C-terminal D,L-endopeptidase domain PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • IseA-LytF (catalytic domain) PubMed

• Localization:
  • secreted (according to Swiss-Prot)
  • localizes to cell septa and poles PubMed

Database entries

• BsubCyc: BSU09370

• Structure:

• UniProt: O07532

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: lytF PubMed

• Expression browser: lytF PubMed

• Sigma factor: SigD PubMed

• Regulation:

• Regulatory mechanism:
  • SlrR (in complex with SinR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A791 ( lytF::spec), PubMed, available at BGSC
  • 1A792 ( lytF::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Original publications

Jeffrey Meisner, Paula Montero Llopis, Lok-To Sham, Ethan Garner, Thomas G Bernhardt, David Z Rudner
FtsEX is required for CwlO peptidoglycan hydrolase activity during cell wall elongation in Bacillus subtilis.
Mol Microbiol: 2013, 89(6);1069-83
[PubMed:23855774] [WorldCat.org] [DOI] (I p)

Ryoichi Arai, Sadaharu Fukui, Naoya Kobayashi, Junichi Sekiguchi
Solution structure of IseA, an inhibitor protein of DL-endopeptidases from Bacillus subtilis, reveals a novel fold with a characteristic inhibitory loop.
J Biol Chem: 2012, 287(53);44736-48
[PubMed:23091053] [WorldCat.org] [DOI] (I p)

Masayuki Hashimoto, Seika Ooiwa, Junichi Sekiguchi
Synthetic lethality of the lytE cwlO genotype in Bacillus subtilis is caused by lack of D,L-endopeptidase activity at the lateral cell wall.
J Bacteriol: 2012, 194(4);796-803
[PubMed:22139507] [WorldCat.org] [DOI] (I p)

Yunrong Chai, Thomas Norman, Roberto Kolter, Richard Losick
An epigenetic switch governing daughter cell separation in Bacillus subtilis.
Genes Dev: 2010, 24(8);754-65
[PubMed:20351052] [WorldCat.org] [DOI] (I p)

Rui Chen, Sarah B Guttenplan, Kris M Blair, Daniel B Kearns
Role of the sigmaD-dependent autolysins in Bacillus subtilis population heterogeneity.
J Bacteriol: 2009, 191(18);5775-84
[PubMed:19542270] [WorldCat.org] [DOI] (I p)

Hiroki Yamamoto, Yukiko Miyake, Miharu Hisaoka, Shin-Ichirou Kurosawa, Junichi Sekiguchi
The major and minor wall teichoic acids prevent the sidewall localization of vegetative DL-endopeptidase LytF in Bacillus subtilis.
Mol Microbiol: 2008, 70(2);297-310
[PubMed:18761696] [WorldCat.org] [DOI] (I p)

Hiroki Yamamoto, Shin-ichirou Kurosawa, Junichi Sekiguchi
Localization of the vegetative cell wall hydrolases LytC, LytE, and LytF on the Bacillus subtilis cell surface and stability of these enzymes to cell wall-bound or extracellular proteases.
J Bacteriol: 2003, 185(22);6666-77
[PubMed:14594841] [WorldCat.org] [DOI] (P p)

R Ohnishi, S Ishikawa, J Sekiguchi
Peptidoglycan hydrolase LytF plays a role in cell separation with CwlF during vegetative growth of Bacillus subtilis.
J Bacteriol: 1999, 181(10);3178-84
[PubMed:10322020] [WorldCat.org] [DOI] (P p)

Philippe Margot, Marco Pagni, Dimitri Karamata
Bacillus subtilis 168 gene lytF encodes a gamma-D-glutamate-meso-diaminopimelate muropeptidase expressed by the alternative vegetative sigma factor, sigmaD.
Microbiology (Reading): 1999, 145 ( Pt 1);57-65
[PubMed:10206711] [WorldCat.org] [DOI] (P p)