ParC

• Description: subunit of DNA topoisomerase IV
  
  

Gene name	parC
Synonyms	grlA
Essential	yes PubMed
Product	subunit of DNA topoisomerase IV
Function	chromosome segregation and compaction
Gene expression levels in SubtiExpress: parC
MW, pI	91 kDa, 6.307
Gene length, protein length	2418 bp, 806 aa
Immediate neighbours	parE, ynfC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA condensation/ segregation, essential genes

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

• Locus tag: BSU18100

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU18100

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: topoisomerase gyrA/parC subunit family (according to Swiss-Prot)

• Paralogous protein(s): GyrA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (Homogeneous) PubMed
  • polar localization in actively growing cells PubMed

Database entries

• BsubCyc: BSU18100

• Structure: 1WP5 (C-terminal domain, Geobacillus stearothermophilus)

• UniProt: Q45066

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: parE-parC PubMed

• Expression browser: parC PubMed

• Sigma factor:

• Regulation:
  • induced by DNA damage (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 67 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

Serkalem Tadesse, Judita Mascarenhas, Bernd Kösters, Andrej Hasilik, Peter L Graumann
Genetic interaction of the SMC complex with topoisomerase IV in Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 11);3729-3737
[PubMed:16272394] [WorldCat.org] [DOI] (P p)

Nora Au, Elke Kuester-Schoeck, Veena Mandava, Laura E Bothwell, Susan P Canny, Karen Chachu, Sierra A Colavito, Shakierah N Fuller, Eli S Groban, Laura A Hensley, Theresa C O'Brien, Amish Shah, Jessica T Tierney, Louise L Tomm, Thomas M O'Gara, Alexi I Goranov, Alan D Grossman, Charles M Lovett
Genetic composition of the Bacillus subtilis SOS system.
J Bacteriol: 2005, 187(22);7655-66
[PubMed:16267290] [WorldCat.org] [DOI] (P p)

Tung-Ju Hsieh, Lynn Farh, Wai Mun Huang, Nei-Li Chan
Structure of the topoisomerase IV C-terminal domain: a broken beta-propeller implies a role as geometry facilitator in catalysis.
J Biol Chem: 2004, 279(53);55587-93
[PubMed:15466871] [WorldCat.org] [DOI] (P p)

W M Huang, J L Libbey, P van der Hoeven, S X Yu
Bipolar localization of Bacillus subtilis topoisomerase IV, an enzyme required for chromosome segregation.
Proc Natl Acad Sci U S A: 1998, 95(8);4652-7
[PubMed:9539793] [WorldCat.org] [DOI] (P p)