Prs

• Description: phosphoribosylpyrophosphate synthetase, universally conserved protein
  
  

Gene name	prs
Synonyms
Essential	yes PubMed
Product	phosphoribosylpyrophosphate synthetase
Function	phosphoribosylpyrophosphate synthesis (biosynthesis of histidine)
Gene expression levels in SubtiExpress: prs
Interactions involving this protein in SubtInteract: Prs
Metabolic function and regulation of this protein in SubtiPathways: prs
MW, pI	34 kDa, 5.895
Gene length, protein length	951 bp, 317 aa
Immediate neighbours	gcaD, ctc
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, biosynthesis/ acquisition of nucleotides, essential genes, universally conserved proteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU00510

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU00510

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate (according to Swiss-Prot)

• Protein family: ribose-phosphate pyrophosphokinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:
  • subject to feedback inhibition by two end products of purine biosynthesis, adenosine 5'-diphosphate (ADP) and guanosine 5'-diphosphate (GDP) PubMed

• Interactions: PurR-Prs

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU00510

• Structure: 1IBS 1DKR1DKU

• UniProt: P14193

• KEGG entry: [3]

• E.C. number: 2.7.6.1

Additional information

universally conserved protein

Expression and regulation

• Operon: gcaD-prs-ctc PubMed

• Expression browser: prs PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1705 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 7417 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: