ThrS

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  • Description: threonyl-tRNA synthetase (major)

Gene name thrS
Synonyms
Essential no
Product threonyl-tRNA synthetase (major)
Function translation
Gene expression levels in SubtiExpress: thrS
Metabolic function and regulation of this protein in SubtiPathways:
thrS
MW, pI 73 kDa, 5.214
Gene length, protein length 1929 bp, 643 aa
Immediate neighbours ysaA, ytxC
Sequences Protein DNA DNA_with_flanks
Genetic context
ThrS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ThrS expression.png















Categories containing this gene/protein

translation, most abundant proteins

This gene is a member of the following regulons

T-box

The gene

Basic information

  • Locus tag: BSU28950

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr) (according to Swiss-Prot)
  • Protein family: class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Paralogous protein(s): ThrZ, one of the two proteins has to be present for viability PubMed

Extended information on the protein

  • Kinetic information:
  • Modification:Cys573 is S-bacillithiolated by NaOCl stress PubMed
  • Effectors of protein activity:

Database entries

  • Structure: 1TJE (from Escherichia coli, 44% identity, 63% similarity) PubMed
  • KEGG entry: [3]

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Regulation:
    • induced by threonine limitation (T-box) PubMed
    • expression transiently increases in the forespore PubMed
  • Additional information:
    • subject to Clp-dependent proteolysis upon glucose starvation PubMed
    • belongs to the 100 most abundant proteins PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 798 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 3009 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References