PlsX

• Description: acyl-acyl carrier protein (ACP):phosphate acyltransferase, catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4), coordinates membrane synthesis with cell division
  
  

Gene name	plsX
Synonyms	ylpD
Essential	yes PubMed
Product	acyl-acyl carrier protein (ACP):phosphate acyltransferase
Function	biosynthesis of phospholipids
Gene expression levels in SubtiExpress: plsX
Interactions involving this protein in SubtInteract: PlsX
Metabolic function and regulation of this protein in SubtiPathways: plsX
MW, pI	35 kDa, 5.492
Gene length, protein length	999 bp, 333 aa
Immediate neighbours	fapR, fabD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell division, biosynthesis of lipids, essential genes

This gene is a member of the following regulons

ComA regulon, FapR regulon

The gene

Basic information

• Locus tag: BSU15890

Phenotypes of a mutant

• essential PubMed
• depletion results in sporulation deficiency (via SpoIIR) PubMed
• a ts mutant forms an abberant Z-ring and has a cell division defect PubMed

Database entries

• BsubCyc: BSU15890

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4)

• Protein family: plsX family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • PlsX-PlsY PubMed
  • PlsX-FtsA PubMed

• Localization:
  • localizes to the cell division sites, colocalizes with the Z-ring PubMed

Database entries

• BsubCyc: BSU15890

• Structure: 1VI1

• UniProt: P71018

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: fapR-plsX-fabD-fabG-acpA PubMed

• Expression browser: plsX PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced if the cells experience an accumulation of malonyl-CoA (FapR) PubMed
  • expressed at high cell density (ComA) PubMed
  • induced upon fatty acid biosynthesis inhibition PubMed

• Regulatory mechanism:
  • ComA: transcription activation (ComA) PubMed
  • FapR: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 237 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 743 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Matsumoto lab PubMed

• Antibody:

References

Hiraku Takada, Sanae Fukushima-Tanaka, Masato Morita, Yasuhiro Kasahara, Satoru Watanabe, Taku Chibazakura, Hiroshi Hara, Kouji Matsumoto, Hirofumi Yoshikawa
An essential enzyme for phospholipid synthesis associates with the Bacillus subtilis divisome.
Mol Microbiol: 2014, 91(2);242-55
[PubMed:24224907] [WorldCat.org] [DOI] (I p)

Veronica Diez, Gustavo E Schujman, Frederico J Gueiros-Filho, Diego de Mendoza
Vectorial signalling mechanism required for cell-cell communication during sporulation in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);261-74
[PubMed:22111992] [WorldCat.org] [DOI] (I p)

Michaela Wenzel, Malay Patra, Dirk Albrecht, David Y-K Chen, K C Nicolaou, Nils Metzler-Nolte, Julia E Bandow
Proteomic signature of fatty acid biosynthesis inhibition available for in vivo mechanism-of-action studies.
Antimicrob Agents Chemother: 2011, 55(6);2590-6
[PubMed:21383089] [WorldCat.org] [DOI] (I p)

Mariano A Martinez, Diego de Mendoza, Gustavo E Schujman
Transcriptional and functional characterization of the gene encoding acyl carrier protein in Bacillus subtilis.
Microbiology (Reading): 2010, 156(Pt 2);484-495
[PubMed:19850612] [WorldCat.org] [DOI] (I p)

Yoshinori Hara, Masahide Seki, Satoshi Matsuoka, Hiroshi Hara, Atsushi Yamashita, Kouji Matsumoto
Involvement of PlsX and the acyl-phosphate dependent sn-glycerol-3-phosphate acyltransferase PlsY in the initial stage of glycerolipid synthesis in Bacillus subtilis.
Genes Genet Syst: 2008, 83(6);433-42
[PubMed:19282621] [WorldCat.org] [DOI] (P p)

Mika Yoshimura, Taku Oshima, Naotake Ogasawara
Involvement of the YneS/YgiH and PlsX proteins in phospholipid biosynthesis in both Bacillus subtilis and Escherichia coli.
BMC Microbiol: 2007, 7;69
[PubMed:17645809] [WorldCat.org] [DOI] (I e)

Luciana Paoletti, Ying-Jie Lu, Gustavo E Schujman, Diego de Mendoza, Charles O Rock
Coupling of fatty acid and phospholipid synthesis in Bacillus subtilis.
J Bacteriol: 2007, 189(16);5816-24
[PubMed:17557823] [WorldCat.org] [DOI] (P p)

Gustavo E Schujman, Marcelo Guerin, Alejandro Buschiazzo, Francis Schaeffer, Leticia I Llarrull, Georgina Reh, Alejandro J Vila, Pedro M Alzari, Diego de Mendoza
Structural basis of lipid biosynthesis regulation in Gram-positive bacteria.
EMBO J: 2006, 25(17);4074-83
[PubMed:16932747] [WorldCat.org] [DOI] (P p)

Natalia Comella, Alan D Grossman
Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis.
Mol Microbiol: 2005, 57(4);1159-74
[PubMed:16091051] [WorldCat.org] [DOI] (P p)

Gustavo E Schujman, Luciana Paoletti, Alan D Grossman, Diego de Mendoza
FapR, a bacterial transcription factor involved in global regulation of membrane lipid biosynthesis.
Dev Cell: 2003, 4(5);663-72
[PubMed:12737802] [WorldCat.org] [DOI] (P p)

H R Morbidoni, D de Mendoza, J E Cronan
Bacillus subtilis acyl carrier protein is encoded in a cluster of lipid biosynthesis genes.
J Bacteriol: 1996, 178(16);4794-800
[PubMed:8759840] [WorldCat.org] [DOI] (P p)