GrpE

• Description: heat-shock protein (activation of DnaK)
  
  

Gene name	grpE
Synonyms
Essential	no
Product	heat-shock protein
Function	control of DnaK activity
Gene expression levels in SubtiExpress: grpE
Metabolic function and regulation of this protein in SubtiPathways: grpE
MW, pI	21 kDa, 4.264
Gene length, protein length	561 bp, 187 aa
Immediate neighbours	dnaK, hrcA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

chaperones/ protein folding, heat shock proteins

This gene is a member of the following regulons

HrcA regulon

The gene

Basic information

• Locus tag: BSU25480

Database entries

• BsubCyc: BSU25480

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: grpE family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: Cytoplasm (Homogeneous) PubMed

Database entries

• BsubCyc: BSU25480

• Structure:

• UniProt: P15874

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: hrcA-grpE-dnaK-dnaJ-yqeT-yqeU-yqeV PubMed

• Expression browser: grpE PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by heat shock (HrcA) PubMed

• Regulatory mechanism:
  • HrcA: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 735 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1765 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Wolfgang Schumann, Bayreuth University, Germany Homepage