DhbE

• Description: 2,3-dihydroxybenzoate-AMP ligase (enterobactin synthetase component E)
  
  

Gene name	dhbE
Synonyms	entE
Essential	no
Product	2,3-dihydroxybenzoate-AMP ligase (enterobactin synthetase component E)
Function	biosynthesis of the siderophore bacillibactin
Gene expression levels in SubtiExpress: dhbE
Metabolic function and regulation of this protein in SubtiPathways: DhbE
MW, pI	59 kDa, 5.684
Gene length, protein length	1617 bp, 539 aa
Immediate neighbours	dhbB, dhbC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

acquisition of iron, iron metabolism

This gene is a member of the following regulons

AbrB regulon, Fur regulon

The gene

Basic information

• Locus tag: BSU31980

Database entries

• BsubCyc: BSU31980

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU31980

• Structure: 1MDB (complex with DHB-adenylate), 1MDF

• UniProt: P40871

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: dhbA-dhbC-dhbE-dhbB-dhbF PubMed

• Expression browser: dhbE PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by iron starvation (Fur) PubMed

• Regulatory mechanism:
  • Fur: transcription repression PubMed
  • AbrB: transcription repression PubMed

• Additional information:
  • the amount of the mRNA is substantially decreased upon depletion of RNase Y PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 4899 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1656 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Mohamed Marahiel, Marburg University, Germany homepage