CadA

• Description: cadmium transporting ATPase, resistance to cadmium
  
  

Gene name	cadA
Synonyms	yvgW
Essential	no
Product	cadmium transporting ATPase
Function	cadmium export
Gene expression levels in SubtiExpress: cadA
Metabolic function and regulation of this protein in SubtiPathways: CadA
MW, pI	75 kDa, 5.998
Gene length, protein length	2106 bp, 702 aa
Immediate neighbours	bdbD, copA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, resistance against toxic metals, membrane proteins

This gene is a member of the following regulons

CzrA regulon

The gene

Basic information

• Locus tag: BSU33490

Database entries

• BsubCyc: BSU33490

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + H₂O + Cd²⁺(In) = ADP + phosphate + Cd²⁺(Out) (according to Swiss-Prot)

• Protein family: Type IB subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU33490

• Structure:
  • ** 4BBJ (CopA from Legionella pneumophila, 32% identity, 67% similarity) PubMed

• UniProt: O32219

• KEGG entry: [2]

• E.C. number: 3.6.3.3

Expression and regulation

• Operon: cadA PubMed

• Expression browser: cadA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by toxic metal ions (Zn(II), Cd(II), Co(II), Ni(II) and Cu(II)) (CzrA) PubMed

• Regulatory mechanism:
  • CzrA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage