TagD
- Description: glycerol-3-phosphate cytidylyltransferase
| Gene name | tagD |
| Synonyms | |
| Essential | yes PubMed |
| Product | glycerol-3-phosphate cytidylyltransferase |
| Function | biosynthesis of teichoic acid |
| Gene expression levels in SubtiExpress: tagD | |
| Metabolic function and regulation of this protein in SubtiPathways: TagD | |
| MW, pI | 15 kDa, 5.672 |
| Gene length, protein length | 387 bp, 129 aa |
| Immediate neighbours | tagE, tagA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
cell wall synthesis, biosynthesis of cell wall components, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU35740
Phenotypes of a mutant
essential PubMed
Database entries
- BsubCyc: BSU35740
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol (according to Swiss-Prot)
- Protein family: cytidylyltransferase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cytoplasm (according to Swiss-Prot)
Database entries
- BsubCyc: BSU35740
- UniProt: P27623
- KEGG entry: [3]
- E.C. number: 2.7.7.39
Additional information
Expression and regulation
- Additional information:
- The mRNA has a long 5' leader region. This may indicate RNA-based regulation PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796]
[WorldCat.org]
[DOI]
(I p)
Alistair Howell, Sarah Dubrac, Kasper Krogh Andersen, David Noone, Juliette Fert, Tarek Msadek, Kevin Devine
Genes controlled by the essential YycG/YycF two-component system of Bacillus subtilis revealed through a novel hybrid regulator approach.
Mol Microbiol: 2003, 49(6);1639-55
[PubMed:12950927]
[WorldCat.org]
[DOI]
(P p)
A P Bhavsar, T J Beveridge, E D Brown
Precise deletion of tagD and controlled depletion of its product, glycerol 3-phosphate cytidylyltransferase, leads to irregular morphology and lysis of Bacillus subtilis grown at physiological temperature.
J Bacteriol: 2001, 183(22);6688-93
[PubMed:11673441]
[WorldCat.org]
[DOI]
(P p)
C H Weber, Y S Park, S Sanker, C Kent, M L Ludwig
A prototypical cytidylyltransferase: CTP:glycerol-3-phosphate cytidylyltransferase from bacillus subtilis.
Structure: 1999, 7(9);1113-24
[PubMed:10508782]
[WorldCat.org]
[DOI]
(P p)
Y Qi, F M Hulett
Role of Pho-P in transcriptional regulation of genes involved in cell wall anionic polymer biosynthesis in Bacillus subtilis.
J Bacteriol: 1998, 180(15);4007-10
[PubMed:9683503]
[WorldCat.org]
[DOI]
(P p)
W Liu, S Eder, F M Hulett
Analysis of Bacillus subtilis tagAB and tagDEF expression during phosphate starvation identifies a repressor role for PhoP-P.
J Bacteriol: 1998, 180(3);753-8
[PubMed:9457886]
[WorldCat.org]
[DOI]
(P p)
C Mauël, A Bauduret, C Chervet, S Beggah, D Karamata
In Bacillus subtilis 168, teichoic acid of the cross-wall may be different from that of the cylinder: a hypothesis based on transcription analysis of tag genes.
Microbiology (Reading): 1995, 141 ( Pt 10);2379-89
[PubMed:7581998]
[WorldCat.org]
[DOI]
(P p)
A L Honeyman, G C Stewart
The nucleotide sequence of the rodC operon of Bacillus subtilis.
Mol Microbiol: 1989, 3(9);1257-68
[PubMed:2507871]
[WorldCat.org]
[DOI]
(P p)
