DnaC

• Description: replicative DNA helicase, part of the replisome
  
  

Gene name	dnaC
Synonyms
Essential	yes PubMed
Product	replicative DNA helicase
Function	DNA replication
Gene expression levels in SubtiExpress: dnaC
Interactions involving this protein in SubtInteract: DnaC
MW, pI	50 kDa, 4.548
Gene length, protein length	1362 bp, 454 aa
Immediate neighbours	yycE, yycD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication, essential genes

The gene

Basic information

• Locus tag: BSU40440

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU40440

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • stimulates DnaG primase activity PubMed

• Protein family: DnaB subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • DnaB-DnaC PubMed
  • DnaI-DnaC PubMed
  • part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
  • YqaM-DnaC PubMed
  • part of the primosome: DnaA-DnaG-DnaC-DnaI-DnaD-DnaB PubMed
  • DnaX-DnaC PubMed
  • DnaE-DnaC PubMed
  • DnaG-DnaC PubMed

• Localization: Cytoplasm (Homogeneous) PubMed

Database entries

• BsubCyc: BSU40440

• Structure:
  • 2R6A (Geobacillus stearothermophilus), 2R6C, 2R6D, 2R6E
  • 4M4W (the DnaC-DnaI-DnaG complex) PubMed

• UniProt: P37469

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: dnaC (according to DBTBS)

• Expression browser: dnaC PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Geoffrey S Briggs, Wiep Klaas Smits, Panos Soultanas
Chromosomal replication initiation machinery of low-G+C-content Firmicutes.
J Bacteriol: 2012, 194(19);5162-70
[PubMed:22797751] [WorldCat.org] [DOI] (I p)