YfmL

• Description: DEAD-box RNA helicase
  
  

Gene name	yfmL
Synonyms
Essential	no
Product	DEAD-box RNA helicase
Function	RNA helicase
Gene expression levels in SubtiExpress: yfmL
MW, pI	42 kDa, 9.602
Gene length, protein length	1128 bp, 376 aa
Immediate neighbours	yfmM, yfmK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DEAD-box RNA helicases, translation

The gene

Basic information

• Locus tag: BSU07430

Phenotypes of a mutant

• poor growth at low temperatures (16 to 20°C) PubMed

Database entries

• BsubCyc: BSU07430

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: RNA helicase

• Protein family: helicase C-terminal domain (according to Swiss-Prot) DEAD-box RNA helicase
• Paralogous protein(s): CshA, CshB, DeaD

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU07430

• Structure:

• UniProt: O34750

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yfmL (according to DBTBS)

• Expression browser: yfmL PubMed

• Sigma factor:

• Regulation:
  • expression declines in the stationary phase PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP1053 (erm), available in the Stülke lab

• Expression vector:
  • for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1069 (spc), available in Jörg Stülke's lab
• lacZ fusion:

• GFP fusion:
  • B. subtilis GP1082 yfmL-yfp spc, available in Jörg Stülke's lab

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

• FLAG-tag construct:
  • GP1066 (spc, based on pGP1331), available in Jörg Stülke's

• Antibody:

References

Martin Lehnik-Habrink, Leonie Rempeters, Ákos T Kovács, Christoph Wrede, Claudia Baierlein, Heike Krebber, Oscar P Kuipers, Jörg Stülke
DEAD-Box RNA helicases in Bacillus subtilis have multiple functions and act independently from each other.
J Bacteriol: 2013, 195(3);534-44
[PubMed:23175651] [WorldCat.org] [DOI] (I p)