deaD

deaD
168

DEAD-box RNA helicase

Locus
BSU_39110
Molecular weight
53.85 kDa
Isoelectric point
7.71
Protein length
479 aaSequenceBlast
Gene length
1440 bpSequenceBlast
Function
RNA helicase
Product
DEAD-box RNA helicase
Essential
no
Synonyms
deaD, yxiN
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0513 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,015,987 → 4,017,426
The protein
Catalyzed reaction/ biological activity
RNA helicase
ATP + H2O --> ADP + H+ + phosphate (according to UniProt)
Protein family
helicase family (according to UniProt)
DEAD-box RNA helicases (according to UniProt)
Domains
Helicase ATP-binding domain (aa 33-203) (according to UniProt)
Helicase C-terminal domain (aa 214-374) (according to UniProt)
Structure
5IVL (PDB) (from Geobacillus stearothermophilus, corresponds to aa 1 ... 373, 44% identity) PubMed
2G0C (PDB) (RNA-binding domain, AA 404-479)
2HJV (PDB) (second domain, AA 207-368)
3MOJ (PDB) (RNA binding domain complexed with a fragment of 23S ribosomal RNA) PubMed
P42305 (AlphaFold)
Paralogous protein(s)
YfmL, CshB, CshA
Expression and Regulation
Operons
Genes
yxiM-deaD
Description
PubMed
Regulation
expression declines in the stationary phase PubMed
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yxiM→deaD

2025-02-25 09:06:13

ghost

82

9114404b986dd658be40735aacff4f346e5ffa25

FC399D6FCA572B3C18E07C665A52D80D4E2B14F1

Biological materials
Mutant
MGNA-B720 (deaD::erm), available at the NBRP B. subtilis, Japan
GP1052 (ΔdeaD::tet), available in Jörg Stülke's lab
BKE39110 (ΔdeaD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTATAAACTCCTGTCTC,  downstream forward: _UP4_AAATGATGAATGACCTGCTC
BKK39110 (ΔdeaD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTATAAACTCCTGTCTC,  downstream forward: _UP4_AAATGATGAATGACCTGCTC
Expression vectors
for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1065 (spc), available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab, PubMed
FLAG-tag construct
GP1068 (spc, based on pGP1331), available in Jörg Stülke's lab
Labs working on this gene/protein
Dagmar Klostermeier, Biozentrum Basel, Switzerland homepage
References
Reviews
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Original Publications
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EACD0C84AA3CE41EF05EAA3D25AA1153293EB562

Page visits: 5361

Time of last update: 2025-04-01 15:55:41

Author of last update: Jstuelk