NasD

• Description: assimilatory nitrite reductase (subunit)
  
  

Gene name	nasD
Synonyms	nasBC, nirB
Essential	no
Product	assimilatory nitrite reductase (subunit)
Function	utilization of nitrite as nitrogen source
Gene expression levels in SubtiExpress: nasD
Interactions involving this protein in SubtInteract: NasD
Metabolic function and regulation of this protein in SubtiPathways: nasD
MW, pI	88 kDa, 4.948
Gene length, protein length	2415 bp, 805 aa
Immediate neighbours	nasE, nasC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

respiration, utilization of nitrogen sources other than amino acids

This gene is a member of the following regulons

NsrR regulon, ResD regulon, TnrA regulon

The gene

Basic information

• Locus tag: BSU03300

Database entries

• BsubCyc: BSU03300

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Ammonium hydroxide + 3 NAD(P)⁺ + H₂O = nitrite + 3 NAD(P)H (according to Swiss-Prot)

• Protein family: nitrite and sulfite reductase 4Fe-4S domain family (according to Swiss-Prot)

• Paralogous protein(s): NasB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:
  • Fe-S cluster PubMed
  • FAD PubMed

• Effectors of protein activity:

• Interactions: NasD-NasE PubMed

• Localization:

Database entries

• BsubCyc: BSU03300

• Structure:

• UniProt: P42435

• KEGG entry: [3]

• E.C. number: 1.7.1.4

Expression and regulation

• Operon:
  • nasB-nasC-nasD-nasE-nasF PubMed
  • nasD-nasE-nasF PubMed

• Expression browser: nasD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • nasD: expressed under anaerobic conditions (ResD) PubMed
  • induced by nitric oxide under anaerobic conditions (NsrR) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed
  • ResD: transcription activation PubMed
  • NsrR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A973 ( nasD::phleo), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Víctor M Luque-Almagro, Andrew J Gates, Conrado Moreno-Vivián, Stuart J Ferguson, David J Richardson, M Dolores Roldán
Bacterial nitrate assimilation: gene distribution and regulation.
Biochem Soc Trans: 2011, 39(6);1838-43
[PubMed:22103536] [WorldCat.org] [DOI] (I p)

D J Richardson, B C Berks, D A Russell, S Spiro, C J Taylor
Functional, biochemical and genetic diversity of prokaryotic nitrate reductases.
Cell Mol Life Sci: 2001, 58(2);165-78
[PubMed:11289299] [WorldCat.org] [DOI] (P p)