DisA

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  • Description: DNA integrity scanning protein,diadenylate cyclase, delays sporulation in the case of chromosome damage, the DisA-dependent checkpoint arrests DNA replication during B. subtilis spore outgrowth until the germinating spore's genome is free of damage

Gene name disA
Synonyms yacK
Essential no
Product DNA integrity scanning protein, has diadenylate cyclase activity
Function control of sporulation initiation
Gene expression levels in SubtiExpress: disA
Interactions involving this protein in SubtInteract: DisA
Metabolic function and regulation of this protein in SubtiPathways:
disA
MW, pI 40 kDa, 5.569
Gene length, protein length 1080 bp, 360 aa
Immediate neighbours radA, yacL
Sequences Protein DNA DNA_with_flanks
Genetic context
YacK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
DisA expression.png















Categories containing this gene/protein

cell division, metabolism of signalling nucleotides, sporulation/ other, general stress proteins (controlled by SigB), cell envelope stress proteins (controlled by SigM, V, W, X, Y), heat shock proteins

This gene is a member of the following regulons

CtsR regulon, SigB regulon, SigM regulon

The gene

Basic information

  • Locus tag: BSU00880

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • synthesis of c-di-AMP from two molecules of ATP PubMed
  • Protein family: disA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:
  • Localization:
    • see a video showing the movement of DisA in the cell (in real time) PubMed
    • forms discrete globular foci in germinating spres that colocalize with the nucleoid PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism: CtsR: transcription repression PubMed
  • Additional information:

Biological materials

  • Expression vector:
    • IPTG inducible expression of His-disA in E. coli: pGP2563 (in pET19b), available in Jörg Stülke's lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

Ute Römling
Great times for small molecules: c-di-AMP, a second messenger candidate in Bacteria and Archaea.
Sci Signal: 2008, 1(33);pe39
[PubMed:18714086] [WorldCat.org] [DOI] (I e)

Original publications