YwrA

• Description: subunit of chromate exporter (with YwrB)
  
  

Gene name	ywrA
Synonyms	chr3C
Essential	no
Product	chromate exporter
Function	chromate detoxification
Gene expression levels in SubtiExpress: ywrA
Interactions involving this protein in SubtInteract: YwrA
MW, pI	19 kDa, 9.94
Gene length, protein length	534 bp, 178 aa
Immediate neighbours	ywrB, ywqO
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, resistance against toxic metals, membrane proteins

This gene is a member of the following regulons

ChrS regulon

The gene

Basic information

• Locus tag: BSU36130

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: YwrA-YwrB PubMed

• Localization:
  • cell membrane PubMed

Database entries

• Structure:

• UniProt: O05215

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: chrS-ywrB-ywrA PubMed

• Expression browser: ywrA PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:
  • ChrS: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Esther Aguilar-Barajas, Selene Jacobo-Arreola, Luis A Verduzco-Rosas, Rafael Jiménez-Mejía, Martha I Ramírez-Díaz, Adriana Julián-Sánchez, Héctor Riveros-Rosas, Carlos Cervantes
An Lrp-type transcriptional regulator controls expression of the Bacillus subtilis chromate transporter.
Antonie Van Leeuwenhoek: 2013, 104(6);941-8
[PubMed:23989926] [WorldCat.org] [DOI] (I p)

Rene Martínez-Valencia, Guadalupe Reyes-Cortés, Martha I Ramírez-Díaz, Héctor Riveros-Rosas, Carlos Cervantes
Antiparallel membrane topology of paired short-chain chromate transport proteins in Bacillus subtilis.
FEMS Microbiol Lett: 2012, 336(2);113-21
[PubMed:22900751] [WorldCat.org] [DOI] (I p)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Amada Díaz-Magaña, Esther Aguilar-Barajas, Rafael Moreno-Sánchez, Martha I Ramírez-Díaz, Héctor Riveros-Rosas, Eréndira Vargas, Carlos Cervantes
Short-chain chromate ion transporter proteins from Bacillus subtilis confer chromate resistance in Escherichia coli.
J Bacteriol: 2009, 191(17);5441-5
[PubMed:19581367] [WorldCat.org] [DOI] (I p)