Sandbox
Revision as of 14:26, 11 November 2013 by 134.76.70.252 (talk)
- Description: DNA polymerase III (beta subunit), beta clamp, part of the replisome, also involved in DNA mismatch repair, inhibitor of DnaA oligomerization
Gene name | dnaN |
Synonyms | dnaG, dnaK |
Essential | yes PubMed |
Product | DNA polymerase III (beta subunit), beta clamp |
Function | DNA replication, DNA repair |
Gene expression levels in SubtiExpress: DnaN | |
Interactions involving this protein in SubtInteract: DnaN | |
MW, pI | 41 kDa, 4.718 |
Gene length, protein length | 1134 bp, 378 aa |
Immediate neighbours | dnaA, yaaA |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
DNA replication, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU00020
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- DnaN overexpression or release from the replisome decreases association of YabA with oriC, increases association of DnaA with oriC PubMed
- Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
- recruits MutS to the site of DNA replication (replisome) PubMed
- required for bacteriophage SPP1 replication PubMed
- inhibits oligomerization and helix formation of DnaA PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- DnaA-YabA-DnaN PubMed
- part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
- PolY1-DnaN, PolY2-DnaN (required for untargeted mutagenesis) PubMed
- PolY1-PolA-DnaN PubMed
- MutL (C-terminal domain)-DnaN PubMed
- MutS-DnaN PubMed
- DnaE-DnaN PubMed
- DnaN forms dimers PubMed
Database entries
- Structure:
- UniProt: P05649
- KEGG entry: [3]
- E.C. number: 2.7.7.7
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Philippe Noirot, Jouy-en-Josas, France homepage
Your additional remarks
References
Reviews
Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559]
[WorldCat.org]
[DOI]
(I p)
Tsutomu Katayama, Shogo Ozaki, Kenji Keyamura, Kazuyuki Fujimitsu
Regulation of the replication cycle: conserved and diverse regulatory systems for DnaA and oriC.
Nat Rev Microbiol: 2010, 8(3);163-70
[PubMed:20157337]
[WorldCat.org]
[DOI]
(I p)
Original publications