PyrG

• Description: CTP synthase (NH3, glutamine)
  
  

Gene name	pyrG
Synonyms	ctrA
Essential	yes PubMed
Product	CTP synthase (NH3, glutamine)
Function	pyrimidine biosynthesis
Gene expression levels in SubtiExpress: pyrG
Metabolic function and regulation of this protein in SubtiPathways: Pyrimidines, Nucleotides (regulation)
MW, pI	59 kDa, 5.16
Gene length, protein length	1605 bp, 535 aa
Immediate neighbours	ywjG, rpoE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, essential genes

The gene

Basic information

• Locus tag: BSU37150

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + UTP + NH₃ = ADP + phosphate + CTP (according to Swiss-Prot)

• Protein family: CTP synthase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1VCM (from Thermus thermophilus, 52% identity, 70% similarity) PubMed

• UniProt: P13242

• KEGG entry: [3]

• E.C. number: 6.3.4.2

Expression and regulation

• Operon: pyrG PubMed

• Expression browser: pyrG PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by cytidine nucleotides (RNA switch) PubMed

• Regulatory mechanism:
  • RNA switch: transcription (termination in the presence of cytidine nucleotides) PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Charles L Turnbough
Regulation of gene expression by reiterative transcription.
Curr Opin Microbiol: 2011, 14(2);142-7
[PubMed:21334966] [WorldCat.org] [DOI] (I p)

Charles L Turnbough, Robert L Switzer
Regulation of pyrimidine biosynthetic gene expression in bacteria: repression without repressors.
Microbiol Mol Biol Rev: 2008, 72(2);266-300, table of contents
[PubMed:18535147] [WorldCat.org] [DOI] (I p)

Original publications

Irene E Jensen-MacAllister, Qi Meng, Robert L Switzer
Regulation of pyrG expression in Bacillus subtilis: CTP-regulated antitermination and reiterative transcription with pyrG templates in vitro.
Mol Microbiol: 2007, 63(5);1440-52
[PubMed:17302819] [WorldCat.org] [DOI] (P p)

Alexander K W Elsholz, Casper Møller Jørgensen, Robert L Switzer
The number of G residues in the Bacillus subtilis pyrG initially transcribed region governs reiterative transcription-mediated regulation.
J Bacteriol: 2007, 189(5);2176-80
[PubMed:17158658] [WorldCat.org] [DOI] (P p)

Qi Meng, Charles L Turnbough, Robert L Switzer
Attenuation control of pyrG expression in Bacillus subtilis is mediated by CTP-sensitive reiterative transcription.
Proc Natl Acad Sci U S A: 2004, 101(30);10943-8
[PubMed:15252202] [WorldCat.org] [DOI] (P p)

Qi Meng, Robert L Switzer
cis-acting sequences of Bacillus subtilis pyrG mRNA essential for regulation by antitermination.
J Bacteriol: 2002, 184(23);6734-8
[PubMed:12426364] [WorldCat.org] [DOI] (P p)

Q Meng, R L Switzer
Regulation of transcription of the Bacillus subtilis pyrG gene, encoding cytidine triphosphate synthetase.
J Bacteriol: 2001, 183(19);5513-22
[PubMed:11544212] [WorldCat.org] [DOI] (P p)

R J Turner, Y Lu, R L Switzer
Regulation of the Bacillus subtilis pyrimidine biosynthetic (pyr) gene cluster by an autogenous transcriptional attenuation mechanism.
J Bacteriol: 1994, 176(12);3708-22
[PubMed:8206849] [WorldCat.org] [DOI] (P p)

C L Quinn, B T Stephenson, R L Switzer
Functional organization and nucleotide sequence of the Bacillus subtilis pyrimidine biosynthetic operon.
J Biol Chem: 1991, 266(14);9113-27
[PubMed:1709162] [WorldCat.org] (P p)

K Trach, J W Chapman, P Piggot, D LeCoq, J A Hoch
Complete sequence and transcriptional analysis of the spo0F region of the Bacillus subtilis chromosome.
J Bacteriol: 1988, 170(9);4194-208
[PubMed:2457578] [WorldCat.org] [DOI] (P p)