AnsA

• Description: L-asparaginase
  
  

Gene name	ansA
Synonyms
Essential	no
Product	L-asparaginase
Function	asparagine degradation
Gene expression levels in SubtiExpress: ansA
Metabolic function and regulation of this protein in SubtiPathways: Asp, Asn
MW, pI	36 kDa, 4.455
Gene length, protein length	987 bp, 329 aa
Immediate neighbours	ansB, ansR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

AnsR regulon

The gene

Basic information

• Locus tag: BSU23580

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-asparagine + H₂O = L-aspartate + NH₃ (according to Swiss-Prot)

• Protein family: asparaginase 1 family (according to Swiss-Prot)

• Paralogous protein(s): AnsZ

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P26900

• KEGG entry: [3]

• E.C. number: 3.5.1.1

Expression and regulation

• Operon:
  • ansA-ansB PubMed
  • ansA-ansB-mleN-mleA PubMed

• Expression browser: ansA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed in the presence of asparagine (AnsR) PubMed

• Regulatory mechanism:
  • AnsR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: GP1153 (del ansAB::ermC) available in the Stülke lab

• Expression vector:

• lacZ fusion: pGP872 (in pAC5), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody: