YngJ

• Description: 3-methylbutanoyl-CoA dehydrogenase
  
  

Gene name	yngJ
Synonyms
Essential	no
Product	3-methylbutanoyl-CoA dehydrogenase
Function	mother cell metabolism, leucine utilization
Gene expression levels in SubtiExpress: yngJ
MW, pI	40 kDa, 5.794
Gene length, protein length	1140 bp, 380 aa
Immediate neighbours	yngI, ynzE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids, sporulation proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

• Locus tag: BSU18260

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 3-methylbutanoyl-CoA --> 3-methylbut-2-enoyl-CoA PubMed

• Protein family:

• Paralogous protein(s): MmgC, AcdA, FadE

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3OWA (from B. anthracis, 37% identity, 54% similarity)

• UniProt: O34421

• KEGG entry: [3]

• E.C. number: 1.3.99.10

Expression and regulation

• Operon: yngJ-yngI-yngH-yngHB-yngG-yngF-yngE PubMed

• Expression browser: yngJ PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed during sporulation in the mother cell (SigE) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Tzu-Lin Hsiao, Olga Revelles, Lifeng Chen, Uwe Sauer, Dennis Vitkup
Automatic policing of biochemical annotations using genomic correlations.
Nat Chem Biol: 2010, 6(1);34-40
[PubMed:19935659] [WorldCat.org] [DOI] (I p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Shane T Jensen, Erin M Conlon, Christiaan van Ooij, Jessica Silvaggi, José Eduardo González-Pastor, Masaya Fujita, Sigal Ben-Yehuda, Patrick Stragier, Jun S Liu, Richard Losick
The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis.
J Mol Biol: 2003, 327(5);945-72
[PubMed:12662922] [WorldCat.org] [DOI] (P p)