RecA

• Description: multifunctional protein involved in homologous recombination and DNA repair (LexA-autocleavage)
  
  

Gene name	recA
Synonyms	recE
Essential	no
Product	multifunctional protein involved in homologous recombination and DNA repair (LexA-autocleavage)
Function	DNA repair/ recombination
Gene expression levels in SubtiExpress: recA
Interactions involving this protein in SubtInteract: RecA
MW, pI	37 kDa, 4.883
Gene length, protein length	1041 bp, 347 aa
Immediate neighbours	cinA, pbpX
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, genetic competence, phosphoproteins

This gene is a member of the following regulons

ComK regulon, LexA regulon

The gene

Basic information

• Locus tag: BSU16940

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

RecA filaments are dismantled from DNA by PcrA PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • RecA stimulates ssDNA phosphorylase activity of PnpA PubMed

• Protein family: recA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-58 PubMed
  • phosphorylated on Ser-2 PubMed by YabT PubMed

• Cofactor(s):

• Effectors of protein activity:
  • RecO and DprA provide RecA access to ssDNA during chromosomal transformation PubMed

• Interactions:
  • RecA-DprA PubMed
  • RecA-RecU PubMed
  • RecA-ComFA PubMed
  • SsbB-RecA PubMed
  • YabT-RecA PubMed

• Localization:
  • cytoplasm (according to Swiss-Prot)
  • Nucleoid (Mid-cell) PubMed
  • localizes to one cell pole PubMed
  • forms a transient, mobile focus associated with the chromosome during spore development PubMed

Database entries

• Structure: 1U94 (RecA from E. coli, 62% identity, 86% similarity)

• UniProt: P16971

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: recA PubMed

• Expression browser: recA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by DNA damage (LexA) PubMed
  • induced by conditions that trigger development of genetic competence (ComK) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:
  • IRN444 (cat), available in Jörg Stülke's lab
  • 1A746 ( recA::erm), PubMed, available at BGSC
  • 1A786 ( recA::kan), PubMed, available at BGSC

• Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Ulf Gerth's lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Peter Graumann, Freiburg University, Germany homepage