SsuD

• Description: aliphatic sulfonate monooxygenase
  
  

Gene name	ssuD
Synonyms	ygcA, yzeC
Essential	no
Product	aliphatic sulfonate monooxygenase
Function	sulfonate reduction
Gene expression levels in SubtiExpress: ssuD
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	41 kDa, 5.369
Gene length, protein length	1128 bp, 376 aa
Immediate neighbours	ssuC, ygaN
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sulfur metabolism

This gene is a member of the following regulons

CymR regulon

The gene

Basic information

• Locus tag: BSU08860

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: An alkanesufonate (R-CH(2)-SO₃H) + FMNH₂ + O₂ = an aldehyde (R-CHO) + FMN + sulfite + H₂O (according to Swiss-Prot)

• Protein family: ssuD family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1NQK (from Escherichia coli, 64% identity, 76% similarity)

• UniProt: P40402

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ssuB-ssuA-ssuC-ssuD-ygaN PubMed

• Expression browser: ssuD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of cysteine (CymR) PubMed

• Regulatory mechanism:
  • CymR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A951 ( ssuD::cat), PubMed, available at BGSC
  • 1A8083 ( ssuD::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: