GlnR

• Description: transcriptional repressor of the glnR-glnA operon
  
  

Gene name	glnR
Synonyms
Essential	no
Product	transcription repressor
Function	regulation of glutamine synthesis
Gene expression levels in SubtiExpress: glnR
Interactions involving this protein in SubtInteract: GlnR
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate, Cell wall, Alternative nitrogen sources
MW, pI	15 kDa, 9.731
Gene length, protein length	405 bp, 135 aa
Immediate neighbours	ynbB, glnA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, glutamate metabolism, transcription factors and their control

This gene is a member of the following regulons

GlnR regulon, TnrA regulon

The GlnR regulon:

• glnR-glnA, tnrA, ureA-ureB-ureC

The gene

Basic information

• Locus tag: BSU17450

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): feedback-inhibited GlnA PubMed

• Effectors of protein activity: activity is enhanced upon interaction of the C-terminal domain with GlnA PubMed

• Interactions:
  • GlnR-GlnA, with feedback-inhibited GlnA, this results in DNA binding PubMed

• Localization:

Database entries

• Structure:

• UniProt: P37582

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: glnR-glnA PubMed

• Expression browser: glnR PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed in the absence of glutamine (GlnR) PubMed
  • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

• Regulatory mechanism:
  • GlnR-GlnA complex: transcription repression PubMed
  • TnrA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion: pGP189 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Susan Fisher, Boston, USA homepage

References

Reviews

Gunka K, Commichau FM    
Control of glutamate homeostasis in Bacillus subtilis: 
a complex interplay between ammonium assimilation,  
glutamate biosynthesis and degradation.  
Mol Microbiol.: 2012, 85(2) 213-224. 
PubMed:22625175