ProH

• Description: pyrroline-5-carboxylate reductase
  
  

Gene name	proH
Synonyms	yoxE
Essential	no
Product	pyrroline-5-carboxylate reductase
Function	osmoadaptive de novo production of proline
Metabolic function and regulation of this protein in SubtiPathways: Pro, Stress
MW, pI	31 kDa, 7.754
Gene length, protein length	891 bp, 297 aa
Immediate neighbours	proJ, rtp
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, coping with hyper-osmotic stress

The gene

Basic information

• Locus tag: BSU18480

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-proline + NAD(P)⁺ = 1-pyrroline-5-carboxylate + NAD(P)H (according to Swiss-Prot)

• Protein family: glutamate 5-kinase family (according to Swiss-Prot)

• Paralogous protein(s): ProI

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P14383

• KEGG entry: [2]

• E.C. number: 1.5.1.2

Expression and regulation

• Operon: proH-proJ PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions of osmotic stress PubMed
  • induced by cold stress PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jeanette Brill, Tamara Hoffmann, Monika Bleisteiner, Erhard Bremer
Osmotically controlled synthesis of the compatible solute proline is critical for cellular defense of Bacillus subtilis against high osmolarity.
J Bacteriol: 2011, 193(19);5335-46
[PubMed:21784929] [WorldCat.org] [DOI] (I p)

Tamara Hoffmann, Erhard Bremer
Protection of Bacillus subtilis against cold stress via compatible-solute acquisition.
J Bacteriol: 2011, 193(7);1552-62
[PubMed:21296969] [WorldCat.org] [DOI] (I p)

B R Belitsky, J Brill, E Bremer, A L Sonenshein
Multiple genes for the last step of proline biosynthesis in Bacillus subtilis.
J Bacteriol: 2001, 183(14);4389-92
[PubMed:11418582] [WorldCat.org] [DOI] (P p)