SsuA

• Description: aliphatic sulfonate ABC transporter (binding lipoprotein)
  
  

Gene name	ssuA
Synonyms	ygbA, yzeA
Essential	no
Product	aliphatic sulfonate ABC transporter (binding lipoprotein)
Function	sulfonate uptake
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	36 kDa, 4.85
Gene length, protein length	996 bp, 332 aa
Immediate neighbours	ssuB, ssuC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU08840

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

ABC transporters, sulfur metabolism, membrane proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: bacterial solute-binding protein ssuA/tauA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: SsuB-SsuC-SsuA PubMed

• Localization: associated to the membrane (via SsuC) PubMed

Database entries

• Structure:

• UniProt: P40400

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ssuB-ssuA-ssuC-ssuD-ygaN PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of cysteine (CymR) PubMed

• Regulatory mechanism:
  • CymR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Sergine Even, Pierre Burguière, Sandrine Auger, Olga Soutourina, Antoine Danchin, Isabelle Martin-Verstraete
Global control of cysteine metabolism by CymR in Bacillus subtilis.
J Bacteriol: 2006, 188(6);2184-97
[PubMed:16513748] [WorldCat.org] [DOI] (P p)

Kyle N Erwin, Shunji Nakano, Peter Zuber
Sulfate-dependent repression of genes that function in organosulfur metabolism in Bacillus subtilis requires Spx.
J Bacteriol: 2005, 187(12);4042-9
[PubMed:15937167] [WorldCat.org] [DOI] (P p)

J R van der Ploeg, M Barone, T Leisinger
Expression of the Bacillus subtilis sulphonate-sulphur utilization genes is regulated at the levels of transcription initiation and termination.
Mol Microbiol: 2001, 39(5);1356-65
[PubMed:11251850] [WorldCat.org] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)

Jan R van der Ploeg, Nicola J Cummings, Thomas Leisinger, Ian F Connerton
Bacillus subtilis genes for the utilization of sulfur from aliphatic sulfonates.
Microbiology (Reading): 1998, 144 ( Pt 9);2555-2561
[PubMed:9782504] [WorldCat.org] [DOI] (P p)