HisC

• Description: histidinol-phosphate aminotransferase / tyrosine and phenylalanine aminotransferase
  
  

Gene name	hisC
Synonyms	aroJ
Essential	no
Product	histidinol-phosphate aminotransferase / tyrosine and phenylalanine aminotransferase
Function	biosynthesis of aromatic amino acids
Metabolic function and regulation of this protein in SubtiPathways: Phe, Tyr, Trp, His
MW, pI	39 kDa, 5.005
Gene length, protein length	1080 bp, 360 aa
Immediate neighbours	tyrA, trpA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context

The gene

Basic information

• Locus tag: BSU22620

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate (according to Swiss-Prot)

• Protein family: bacterial solute-binding protein 3 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1UU0 (the enzyme from E. coli) PubMed

• Swiss prot entry: P17731

• KEGG entry: [3]

• E.C. number: 2.6.1.9

Expression and regulation

• Operon:
  • aroF-aroB-aroH-trpE-trpD-trpC-trpF-trpB-trpA-hisC-tyrA-aroE (according to DBTBS)
  • trpE-trpD-trpC-trpF-trpB-trpA-hisC-tyrA-aroE PubMed
  • hisC-tyrA-aroE (according to DBTBS)

• Sigma factor:
  • trpE: SigA PubMed

• Regulation:
  • induction by tryptophan ( TRAP) PubMed

• Regulatory mechanism:
  • TRAP: binding to the mRNA in the presence of tryptophan, this results in transcription termination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

D A Weigent, E W Nester
Regulation of histidinol phosphate aminotransferase synthesis by tryptophan in Bacillus subtilis.
J Bacteriol: 1976, 128(1);202-11
[PubMed:824269] [WorldCat.org] [DOI] (P p)

E W Nester, A L Montoya
An enzyme common to histidine and aromatic amino acid biosynthesis in Bacillus subtilis.
J Bacteriol: 1976, 126(2);699-705
[PubMed:4431] [WorldCat.org] [DOI] (P p)