CheD

• Description: required for methylation of methyl-accepting chemotaxis proteins by CheR, enhances phosphatase activity of CheC
  
  

Gene name	cheD
Synonyms	ylxK
Essential	no
Product	CheC activity modulator
Function	motility, chemotaxis
MW, pI	17 kDa, 9.347
Gene length, protein length	498 bp, 166 aa
Immediate neighbours	cheC, sigD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU16460

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein L-glutamine + H₂O = protein L-glutamate + NH₃ (according to Swiss-Prot)

• Protein family: cheD family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: CheC-CheD PubMed

• Localization:

Database entries

• Structure:

• UniProt: P40404

• KEGG entry: [3]

• E.C. number: 3.5.1.44

Expression and regulation

• Operon:
  • flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-ylxG-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-ylxH-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed
  • ylxF-fliK-ylxG-flgE-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-ylxH-cheB-cheA-cheW-cheC-cheD-sigD-swrB PubMed

• Sigma factor:
  • flgB: SigA PubMed, SigD PubMed
  • ylxF: SigD PubMed

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed
  • CodY: transcription repression PubMed
  • DegU (non-phosphorylated): transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: