ManR

• Description: transcriptional activator of the manP-manA-yjdF operon
  
  

Gene name	manR
Synonyms	yjdC
Essential	no
Product	transcriptional activator, PRD-type
Function	regulation of mannose utilization
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	75 kDa, 8.04
Gene length, protein length	1944 bp, 648 aa
Immediate neighbours	yjdB, manP
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU12000

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation by ManP inactivates ManR (in the absence of mannose), phosphorylation by HPr stimluates ManR PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions: ManP-ManR, HPr-ManR,

• Localization:

Database entries

• Structure:

• UniProt: O31644

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: manR PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by mannose (ManR) PubMed
  • repressed by glucose (ManR) PubMed

• Regulatory mechanism:
  • ManR: transcription activation in the presence of mannose and absence of glucose PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Tianqi Sun, Josef Altenbuchner
Characterization of a mannose utilization system in Bacillus subtilis.
J Bacteriol: 2010, 192(8);2128-39
[PubMed:20139185] [WorldCat.org] [DOI] (I p)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)

J Stülke, M Arnaud, G Rapoport, I Martin-Verstraete
PRD--a protein domain involved in PTS-dependent induction and carbon catabolite repression of catabolic operons in bacteria.
Mol Microbiol: 1998, 28(5);865-74
[PubMed:9663674] [WorldCat.org] [DOI] (P p)