Cca

• Description: tRNA nucleotidyltransferase, maturation of the single-copy tRNACys, which lacks an encoded CCA 3' end
  
  

Gene name	cca
Synonyms	papS, ypjI
Essential	yes PubMed
Product	tRNA nucleotidyltransferase
Function	tRNA modification
MW, pI	45 kDa, 8.041
Gene length, protein length	1191 bp, 397 aa
Immediate neighbours	birA, bshA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU22450

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + tRNA(n) = diphosphate + tRNA(n+1) (according to Swiss-Prot)

• Protein family: the protein is similar to the E. coli poly(A) polymerase

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1MIV (Geobacillus stearothermophilus 44% identity)

• UniProt: P42977

• KEGG entry: [2]

• E.C. number: 2.7.7.25

Expression and regulation

• Operon: ypjD-dapB-mgsA-bshB1-bshA-cca-birA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Juan Campos-Guillén, Jackeline Lizzeta Arvizu-Gómez, George H Jones, Gabriela Olmedo-Alvarez
Characterization of tRNA(Cys) processing in a conditional Bacillus subtilis CCase mutant reveals the participation of RNase R in its quality control.
Microbiology (Reading): 2010, 156(Pt 7);2102-2111
[PubMed:20360175] [WorldCat.org] [DOI] (I p)

Hyundae D Cho, Christophe L M J Verlinde, Alan M Weiner
Reengineering CCA-adding enzymes to function as (U,G)- or dCdCdA-adding enzymes or poly(C,A) and poly(U,G) polymerases.
Proc Natl Acad Sci U S A: 2007, 104(1);54-9
[PubMed:17179213] [WorldCat.org] [DOI] (P p)

Patricia Bralley, Samantha A Chang, George H Jones
A phylogeny of bacterial RNA nucleotidyltransferases: Bacillus halodurans contains two tRNA nucleotidyltransferases.
J Bacteriol: 2005, 187(17);5927-36
[PubMed:16109934] [WorldCat.org] [DOI] (P p)

Fang Li, Yong Xiong, Jimin Wang, HyunDae D Cho, Kozo Tomita, Alan M Weiner, Thomas A Steitz
Crystal structures of the Bacillus stearothermophilus CCA-adding enzyme and its complexes with ATP or CTP.
Cell: 2002, 111(6);815-24
[PubMed:12526808] [WorldCat.org] [DOI] (P p)

L C Raynal, H M Krisch, A J Carpousis
The Bacillus subtilis nucleotidyltransferase is a tRNA CCA-adding enzyme.
J Bacteriol: 1998, 180(23);6276-82
[PubMed:9829937] [WorldCat.org] [DOI] (P p)

Operon and expression

Ahmed Gaballa, Gerald L Newton, Haike Antelmann, Derek Parsonage, Heather Upton, Mamta Rawat, Al Claiborne, Robert C Fahey, John D Helmann
Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.
Proc Natl Acad Sci U S A: 2010, 107(14);6482-6
[PubMed:20308541] [WorldCat.org] [DOI] (I p)