SpoIVFA

• Description: inhibitor of SpoIVFB metalloprotease
  
  

Gene name	spoIVFA
Synonyms	bofB, spoVL
Essential	no
Product	inhibitor of SpoIVFB metalloprotease
Function	control of SigK activation
MW, pI	29 kDa, 7.292
Gene length, protein length	792 bp, 264 aa
Immediate neighbours	spoIVFB, minD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU27980

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: forespore outer membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P26936

• KEGG entry: [3]

• E.C. number:

Additional information

cleaved by SpoIVB and CtpB

Expression and regulation

• Operon: spoIVFA-spoIVFB PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed early during sporulation in the mother cell (SigE, SpoIIID) PubMed

• Regulatory mechanism:
  • SpoIIID: transcription repression PubMed

• Additional information: cleaved by SpoIVB and CtpB

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: