AppB

• Description: oligopeptide ABC transporter (permease)
  
  

Gene name	appB
Synonyms
Essential	no
Product	oligopeptide ABC transporter (permease))
Function	uptake of oligopeptides
Gene expression levels in SubtiExpress: appB
Interactions involving this protein in SubtInteract: AppB
Metabolic function and regulation of this protein in SubtiPathways: appB
MW, pI	35 kDa, 9.772
Gene length, protein length	951 bp, 317 aa
Immediate neighbours	appA/2, appC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

ABC transporters, utilization of nitrogen sources other than amino acids, membrane proteins

This gene is a member of the following regulons

CodY regulon, ScoC regulon

The gene

Basic information

• Locus tag: BSU11390

Database entries

• BsubCyc: BSU11390

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ABC transmembrane type-1 domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • (AppD-AppF)-(AppB-AppC)-AppA PubMed

• Localization: cell membrane PubMed

Database entries

• BsubCyc: BSU11390

• Structure:

• UniProt: P42062

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: appD-appF-appA-appB-appC PubMed

• Expression browser: appB PubMed

• Sigma factor:

• Regulation:
  • repressed during exponential growth (ScoC) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • ScoC: transcription repression PubMed
  • CodY: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 124 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

A Koide, M Perego, J A Hoch
ScoC regulates peptide transport and sporulation initiation in Bacillus subtilis.
J Bacteriol: 1999, 181(13);4114-7
[PubMed:10383984] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)

A Koide, J A Hoch
Identification of a second oligopeptide transport system in Bacillus subtilis and determination of its role in sporulation.
Mol Microbiol: 1994, 13(3);417-26
[PubMed:7997159] [WorldCat.org] [DOI] (P p)