AraR

• Description: repressor of the arabinose utilization genes
  
  

Gene name	araR
Synonyms	araC, yvbS
Essential	no
Product	transcriptional repressor (GntR family, LacI family)
Function	regulation of arabinose utilization (araABDLMNPQ-abfA) and of the araE/araR genes
Gene expression levels in SubtiExpress: araR (araABDLMNPQ-abfA) and of the araE/araR genes
Metabolic function and regulation of this protein in SubtiPathways: AraR
MW, pI	43 kDa, 6.341
Gene length, protein length	1152 bp, 384 aa
Immediate neighbours	araE, yvbT
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control

This gene is a member of the following regulons

AraR regulon

The gene

Basic information

• Locus tag: BSU33970

Database entries

• BsubCyc: BSU33970

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: GntR family: N-terminal DNA-binding domain, LacI family: C-terminal effector-binding domain

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • N-terminal DNA-binding domain (GntR family)
  • C-terminal effector-binding domain (LacI family)

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU33970

• Structure: 3TB6 (effector-binding domain) PubMed

• UniProt: P96711

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: araR PubMed

• Expression browser: araR PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by arabinose (AraR) PubMed

• Regulatory mechanism:
  • AraR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Deepti Jain, Deepak T Nair
Spacing between core recognition motifs determines relative orientation of AraR monomers on bipartite operators.
Nucleic Acids Res: 2013, 41(1);639-47
[PubMed:23109551] [WorldCat.org] [DOI] (I p)

Irina Saraiva Franco, Luís Jaime Mota, Cláudio Manuel Soares, Isabel de Sá-Nogueira
Probing key DNA contacts in AraR-mediated transcriptional repression of the Bacillus subtilis arabinose regulon.
Nucleic Acids Res: 2007, 35(14);4755-66
[PubMed:17617643] [WorldCat.org] [DOI] (I p)

Irina Saraiva Franco, Luís Jaime Mota, Cláudio Manuel Soares, Isabel de Sá-Nogueira
Functional domains of the Bacillus subtilis transcription factor AraR and identification of amino acids important for nucleoprotein complex assembly and effector binding.
J Bacteriol: 2006, 188(8);3024-36
[PubMed:16585763] [WorldCat.org] [DOI] (P p)

Maria Paiva Raposo, José Manuel Inácio, Luís Jaime Mota, Isabel de Sá-Nogueira
Transcriptional regulation of genes encoding arabinan-degrading enzymes in Bacillus subtilis.
J Bacteriol: 2004, 186(5);1287-96
[PubMed:14973026] [WorldCat.org] [DOI] (P p)

L J Mota, L M Sarmento, I de Sá-Nogueira
Control of the arabinose regulon in Bacillus subtilis by AraR in vivo: crucial roles of operators, cooperativity, and DNA looping.
J Bacteriol: 2001, 183(14);4190-201
[PubMed:11418559] [WorldCat.org] [DOI] (P p)

L J Mota, P Tavares, I Sá-Nogueira
Mode of action of AraR, the key regulator of L-arabinose metabolism in Bacillus subtilis.
Mol Microbiol: 1999, 33(3);476-89
[PubMed:10417639] [WorldCat.org] [DOI] (P p)

I Sá-Nogueira, L J Mota
Negative regulation of L-arabinose metabolism in Bacillus subtilis: characterization of the araR (araC) gene.
J Bacteriol: 1997, 179(5);1598-608
[PubMed:9045819] [WorldCat.org] [DOI] (P p)

I Sá-Nogueira, H Paveia, H de Lencastre
Isolation of constitutive mutants for L-arabinose utilization in Bacillus subtilis.
J Bacteriol: 1988, 170(6);2855-7
[PubMed:3131313] [WorldCat.org] [DOI] (P p)