HemAT

• Description: soluble chemotaxis receptor, heme-containing O(2) sensor protein
  
  

Gene name	hemAT
Synonyms	yhfV
Essential	no
Product	haem-based aerotactic transducer
Function	movement towards oxygen
Gene expression levels in SubtiExpress: hemAT
Interactions involving this protein in SubtInteract: HemAT
MW, pI	48 kDa, 5.441
Gene length, protein length	1296 bp, 432 aa
Immediate neighbours	yhfU, yhfW
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

• Locus tag: BSU10380

Database entries

• BsubCyc: BSU10380

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • required for full expression of the phoD-tatAD-tatCD operon PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): heme

• Effectors of protein activity:

• Interactions:
  • HemAT-TatAC PubMed

• Localization:
  • forms clusters at the cell poles PubMed
  • homogeneous cytoplsmic distribution in chain-forming cells during the logarithmic phase PubMed
  • polar foci in individual cells at later growth stages PubMed

Database entries

• BsubCyc: BSU10380

• Structure: 1OR4 (in cyano-liganded form), 1OR6

• UniProt: O07621

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: hemAT PubMed

• Expression browser: hemAT PubMed

• Sigma factor: SigD PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:
  • in minimal medium, HemAT is present with 19,000 +/- 3,900 molecules per cell PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Markéta Martínková, Kenichi Kitanishi, Toru Shimizu
Heme-based globin-coupled oxygen sensors: linking oxygen binding to functional regulation of diguanylate cyclase, histidine kinase, and methyl-accepting chemotaxis.
J Biol Chem: 2013, 288(39);27702-11
[PubMed:23928310] [WorldCat.org] [DOI] (I p)