AlkA

• Description: DNA-3-methyladenine glycosylase
  
  

Gene name	alkA
Synonyms
Essential	no
Product	DNA-3-methyladenine glycosylase
Function	adaptive response to alkylative DNA damage
Gene expression levels in SubtiExpress: alkA
MW, pI	35 kDa, 8.147
Gene length, protein length	909 bp, 303 aa
Immediate neighbours	glmS, adaA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, prophage 1

This gene is a member of the following regulons

AdaA regulon

The gene

Basic information

• Locus tag: BSU01800

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine (according to Swiss-Prot)

• Protein family: alkylbase DNA glycosidase alkA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P37878

• KEGG entry: [3]

• E.C. number: 3.2.2.21

Expression and regulation

• Operon: alkA PubMed

• Expression browser: alkA PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism: AdaA: positive regulation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

Original publications

F Morohoshi, K Hayashi, N Munkata
Bacillus subtilis alkA gene encoding inducible 3-methyladenine DNA glycosylase is adjacent to the ada operon.
J Bacteriol: 1993, 175(18);6010-7
[PubMed:8376346] [WorldCat.org] [DOI] (P p)