MtrB

• Description: tryptophan operon RNA-binding attenuation protein (TRAP), controls the RNA switch in front of genes involved in biosynthesis and acquisition of tryptophan
  
  

Gene name	mtrB
Synonyms
Essential	no
Product	tryptophan operon RNA-binding attenuation protein (TRAP)
Function	regulation of tryptophan biosynthesis (and translation) attenuation in the trp operon; repression of the folate operon
Gene expression levels in SubtiExpress: mtrB
Interactions involving this protein in SubtInteract: TRAP
Metabolic function and regulation of this protein in SubtiPathways: mtrB
MW, pI	8 kDa, 7.333
Gene length, protein length	225 bp, 75 aa
Immediate neighbours	hepS, folE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, transcription factors and their control, RNA binding regulators

The gene

Basic information

• Locus tag: BSU22770

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• mutations in mtrB, sigB, rpoB, and rpoC allow B. subtilis to grow with 4-fluorotryptophan rather than with tryptophan as a canonical amino acid of the genetic code PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: prpD family (according to Swiss-Prot)

• Paralogous protein(s):

Genes controlled by MtrB

• pabA PubMed
• trpE-trpD-trpC-trpF-trpB-trpA PubMed
• trpP PubMed
• ycbK PubMed

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:
  • binding of tryptophan results RNA binding and thus in transcription termination

• Interactions:
  • forms 11-mers
  • TRAP₁₁-RtpA₃ PubMed

• Localization:

Database entries

• Structure: 3AQD, 1WAP (complex with L-tryptophan), 2ZP9 (complex with RtpA)

• UniProt: P19466

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: folE-mtrB PubMed

• Expression browser: mtrB PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Paul Babitzke, Carol S Baker, Tony Romeo
Regulation of translation initiation by RNA binding proteins.
Annu Rev Microbiol: 2009, 63;27-44
[PubMed:19385727] [WorldCat.org] [DOI] (I p)

Paul Gollnick, Paul Babitzke, Alfred Antson, Charles Yanofsky
Complexity in regulation of tryptophan biosynthesis in Bacillus subtilis.
Annu Rev Genet: 2005, 39;47-68
[PubMed:16285852] [WorldCat.org] [DOI] (P p)

Paul Babitzke
Regulation of transcription attenuation and translation initiation by allosteric control of an RNA-binding protein: the Bacillus subtilis TRAP protein.
Curr Opin Microbiol: 2004, 7(2);132-9
[PubMed:15063849] [WorldCat.org] [DOI] (P p)